Composite
Part:BBa_K4790101:Design
Designed by: Yutong Ji Group: iGEM23_NNU-CHINA (2023-10-11)
ASR1(node 59)-M40E/pET-22b(+)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 960
Illegal PstI site found at 261
Illegal PstI site found at 393 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 960
Illegal NheI site found at 882
Illegal NheI site found at 1113
Illegal PstI site found at 261
Illegal PstI site found at 393 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 960
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 960
Illegal PstI site found at 261
Illegal PstI site found at 393 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 960
Illegal PstI site found at 261
Illegal PstI site found at 393
Illegal NgoMIV site found at 143 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We should select direction of mutation which is predicted the highest possibility. Mutation should refer to Codon preference in E.coli.
Source
It does not come from any genomic sequence. Node 59 comes from Ancestral Sequence Reconstruction (ASR). The mutation site is predicted by machine learning.